Biochemical characterization of isoprene synthase from Ipomoea batatas.
Identifieur interne : 000B05 ( Main/Exploration ); précédent : 000B04; suivant : 000B06Biochemical characterization of isoprene synthase from Ipomoea batatas.
Auteurs : Meijie Li [République populaire de Chine] ; Changqing Liu [République populaire de Chine] ; Hailin Chen [République populaire de Chine] ; Li Deng [République populaire de Chine] ; Haibo Zhang [République populaire de Chine] ; Rui Nian [République populaire de Chine] ; Mo Xian [République populaire de Chine]Source :
- Journal of bioscience and bioengineering [ 1347-4421 ] ; 2019.
Descripteurs français
- KwdFr :
- Alkyl et aryl transferases (composition chimique), Alkyl et aryl transferases (génétique), Alkyl et aryl transferases (isolement et purification), Alkyl et aryl transferases (métabolisme), Butadiènes (métabolisme), Cinétique (MeSH), Clonage moléculaire (MeSH), Escherichia coli (MeSH), Hémiterpènes (métabolisme), Ions lourds (MeSH), Ipomoea batatas (enzymologie), Ipomoea batatas (génétique), Ipomoea batatas (métabolisme), Magnésium (composition chimique), Magnésium (métabolisme), Protéines recombinantes (composition chimique), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), Stabilité enzymatique (MeSH), Température (MeSH).
- MESH :
- composition chimique : Alkyl et aryl transferases, Magnésium, Protéines recombinantes.
- enzymologie : Ipomoea batatas.
- génétique : Alkyl et aryl transferases, Ipomoea batatas.
- isolement et purification : Alkyl et aryl transferases, Protéines recombinantes.
- métabolisme : Alkyl et aryl transferases, Butadiènes, Hémiterpènes, Ipomoea batatas, Magnésium, Protéines recombinantes.
- Cinétique, Clonage moléculaire, Escherichia coli, Ions lourds, Stabilité enzymatique, Température.
English descriptors
- KwdEn :
- Alkyl and Aryl Transferases (chemistry), Alkyl and Aryl Transferases (genetics), Alkyl and Aryl Transferases (isolation & purification), Alkyl and Aryl Transferases (metabolism), Butadienes (metabolism), Cloning, Molecular (MeSH), Enzyme Stability (MeSH), Escherichia coli (MeSH), Heavy Ions (MeSH), Hemiterpenes (metabolism), Ipomoea batatas (enzymology), Ipomoea batatas (genetics), Ipomoea batatas (metabolism), Kinetics (MeSH), Magnesium (chemistry), Magnesium (metabolism), Recombinant Proteins (chemistry), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Temperature (MeSH).
- MESH :
- chemical , chemistry : Alkyl and Aryl Transferases, Magnesium, Recombinant Proteins.
- chemical , genetics : Alkyl and Aryl Transferases.
- chemical , isolation & purification : Alkyl and Aryl Transferases, Recombinant Proteins.
- chemical , metabolism : Alkyl and Aryl Transferases, Butadienes, Hemiterpenes, Magnesium, Recombinant Proteins.
- enzymology : Ipomoea batatas.
- genetics : Ipomoea batatas.
- metabolism : Ipomoea batatas.
- Cloning, Molecular, Enzyme Stability, Escherichia coli, Heavy Ions, Kinetics, Temperature.
Abstract
The bio-production process of isoprene, an essential chemical used in industry, is strongly limited by isoprene synthase. In our previous work, relatively high isoprene production was observed with isoprene synthase from Ipomoea batatas (IspSib). In this work the biochemical properties of IspSib were analyzed and compared with those of isoprene synthase from Populus alba (IspSpa) and other species. Firstly, IspSib and IspSpa were expressed, purified, and identified by SDS-PAGE and western blot analysis. Secondly, pH and temperature dependence of IspSib were performed and an optimum pH of 8.6 and an optimum temperature of 42 °C were resulted. Mg2+ with optimum concentration of 56 mM was proved to be needed for enzyme activation. In addition, in vivo and in vitro study of the thermostabilities of IspSib and IspSpa were performed. The enzyme activity of IspSib and IspSpa dropped very rapidly after incubation at 30 °C; almost 80% enzyme activity of IspSib was lost after 20 min of incubation. Moreover, the Michaelis-Menten constant was measured. IspSib showed a lower Km, 0.2 mM, and a higher kcat, 0.37 s-1, as compared with IspSpa. The high catalytic efficiency, which was reflected by the high kcat/Km ratio, indicates that IspSib is a good candidate for the bio-isoprene production, while its thermal instability remains as a challenge. Enzyme engineering efforts, such as direction evolution or semi-rational evolution, are planned for further research.
DOI: 10.1016/j.jbiosc.2018.07.022
PubMed: 30190176
Affiliations:
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Electronic address: li_mj@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Liu, Changqing" sort="Liu, Changqing" uniqKey="Liu C" first="Changqing" last="Liu">Changqing Liu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: liucq@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chen, Hailin" sort="Chen, Hailin" uniqKey="Chen H" first="Hailin" last="Chen">Hailin Chen</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: chenhl@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Deng, Li" sort="Deng, Li" uniqKey="Deng L" first="Li" last="Deng">Li Deng</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: dengli@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101</wicri:regionArea><wicri:noRegion>Qingdao 266101</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Haibo" sort="Zhang, Haibo" uniqKey="Zhang H" first="Haibo" last="Zhang">Haibo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: zhanghb@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101</wicri:regionArea><wicri:noRegion>Qingdao 266101</wicri:noRegion></affiliation></author><author><name sortKey="Nian, Rui" sort="Nian, Rui" uniqKey="Nian R" first="Rui" last="Nian">Rui Nian</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: nianrui@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101</wicri:regionArea><wicri:noRegion>Qingdao 266101</wicri:noRegion></affiliation></author><author><name sortKey="Xian, Mo" sort="Xian, Mo" uniqKey="Xian M" first="Mo" last="Xian">Mo Xian</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: xianmo1@qibebt.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101</wicri:regionArea><wicri:noRegion>Qingdao 266101</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of bioscience and bioengineering</title><idno type="eISSN">1347-4421</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkyl and Aryl Transferases (chemistry)</term><term>Alkyl and Aryl Transferases (genetics)</term><term>Alkyl and Aryl Transferases (isolation & purification)</term><term>Alkyl and Aryl Transferases (metabolism)</term><term>Butadienes (metabolism)</term><term>Cloning, Molecular (MeSH)</term><term>Enzyme Stability (MeSH)</term><term>Escherichia coli (MeSH)</term><term>Heavy Ions (MeSH)</term><term>Hemiterpenes (metabolism)</term><term>Ipomoea batatas (enzymology)</term><term>Ipomoea batatas (genetics)</term><term>Ipomoea batatas (metabolism)</term><term>Kinetics (MeSH)</term><term>Magnesium (chemistry)</term><term>Magnesium (metabolism)</term><term>Recombinant Proteins (chemistry)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Temperature (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alkyl et aryl transferases (composition chimique)</term><term>Alkyl et aryl transferases (génétique)</term><term>Alkyl et aryl transferases (isolement et purification)</term><term>Alkyl et aryl transferases (métabolisme)</term><term>Butadiènes (métabolisme)</term><term>Cinétique (MeSH)</term><term>Clonage moléculaire (MeSH)</term><term>Escherichia coli (MeSH)</term><term>Hémiterpènes (métabolisme)</term><term>Ions lourds (MeSH)</term><term>Ipomoea batatas (enzymologie)</term><term>Ipomoea batatas (génétique)</term><term>Ipomoea batatas (métabolisme)</term><term>Magnésium (composition chimique)</term><term>Magnésium (métabolisme)</term><term>Protéines recombinantes (composition chimique)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>Stabilité enzymatique (MeSH)</term><term>Température (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Alkyl and Aryl Transferases</term><term>Magnesium</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alkyl and Aryl Transferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Alkyl and Aryl Transferases</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkyl and Aryl Transferases</term><term>Butadienes</term><term>Hemiterpenes</term><term>Magnesium</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Magnésium</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Ipomoea batatas</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Ipomoea batatas</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ipomoea batatas</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Ipomoea batatas</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ipomoea batatas</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Alkyl et aryl transferases</term><term>Butadiènes</term><term>Hémiterpènes</term><term>Ipomoea batatas</term><term>Magnésium</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cloning, Molecular</term><term>Enzyme Stability</term><term>Escherichia coli</term><term>Heavy Ions</term><term>Kinetics</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cinétique</term><term>Clonage moléculaire</term><term>Escherichia coli</term><term>Ions lourds</term><term>Stabilité enzymatique</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The bio-production process of isoprene, an essential chemical used in industry, is strongly limited by isoprene synthase. In our previous work, relatively high isoprene production was observed with isoprene synthase from Ipomoea batatas (IspS<sub>ib</sub>). In this work the biochemical properties of IspS<sub>ib</sub> were analyzed and compared with those of isoprene synthase from Populus alba (IspS<sub>pa</sub>) and other species. Firstly, IspS<sub>ib</sub> and IspS<sub>pa</sub> were expressed, purified, and identified by SDS-PAGE and western blot analysis. Secondly, pH and temperature dependence of IspS<sub>ib</sub> were performed and an optimum pH of 8.6 and an optimum temperature of 42 °C were resulted. Mg<sup>2+</sup> with optimum concentration of 56 mM was proved to be needed for enzyme activation. In addition, in vivo and in vitro study of the thermostabilities of IspS<sub>ib</sub> and IspS<sub>pa</sub> were performed. The enzyme activity of IspS<sub>ib</sub> and IspS<sub>pa</sub> dropped very rapidly after incubation at 30 °C; almost 80% enzyme activity of IspS<sub>ib</sub> was lost after 20 min of incubation. Moreover, the Michaelis-Menten constant was measured. IspS<sub>ib</sub> showed a lower K<sub>m</sub>, 0.2 mM, and a higher k<sub>cat</sub>, 0.37 s<sup>-1</sup>, as compared with IspS<sub>pa</sub>. The high catalytic efficiency, which was reflected by the high k<sub>cat</sub>/K<sub>m</sub> ratio, indicates that IspS<sub>ib</sub> is a good candidate for the bio-isoprene production, while its thermal instability remains as a challenge. Enzyme engineering efforts, such as direction evolution or semi-rational evolution, are planned for further research.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30190176</PMID><DateCompleted><Year>2019</Year><Month>03</Month><Day>07</Day></DateCompleted><DateRevised><Year>2019</Year><Month>03</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4421</ISSN><JournalIssue CitedMedium="Internet"><Volume>127</Volume><Issue>2</Issue><PubDate><Year>2019</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Journal of bioscience and bioengineering</Title><ISOAbbreviation>J Biosci Bioeng</ISOAbbreviation></Journal><ArticleTitle>Biochemical characterization of isoprene synthase from Ipomoea batatas.</ArticleTitle><Pagination><MedlinePgn>138-144</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1389-1723(18)30239-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiosc.2018.07.022</ELocationID><Abstract><AbstractText>The bio-production process of isoprene, an essential chemical used in industry, is strongly limited by isoprene synthase. In our previous work, relatively high isoprene production was observed with isoprene synthase from Ipomoea batatas (IspS<sub>ib</sub>). In this work the biochemical properties of IspS<sub>ib</sub> were analyzed and compared with those of isoprene synthase from Populus alba (IspS<sub>pa</sub>) and other species. Firstly, IspS<sub>ib</sub> and IspS<sub>pa</sub> were expressed, purified, and identified by SDS-PAGE and western blot analysis. Secondly, pH and temperature dependence of IspS<sub>ib</sub> were performed and an optimum pH of 8.6 and an optimum temperature of 42 °C were resulted. Mg<sup>2+</sup> with optimum concentration of 56 mM was proved to be needed for enzyme activation. In addition, in vivo and in vitro study of the thermostabilities of IspS<sub>ib</sub> and IspS<sub>pa</sub> were performed. The enzyme activity of IspS<sub>ib</sub> and IspS<sub>pa</sub> dropped very rapidly after incubation at 30 °C; almost 80% enzyme activity of IspS<sub>ib</sub> was lost after 20 min of incubation. Moreover, the Michaelis-Menten constant was measured. IspS<sub>ib</sub> showed a lower K<sub>m</sub>, 0.2 mM, and a higher k<sub>cat</sub>, 0.37 s<sup>-1</sup>, as compared with IspS<sub>pa</sub>. The high catalytic efficiency, which was reflected by the high k<sub>cat</sub>/K<sub>m</sub> ratio, indicates that IspS<sub>ib</sub> is a good candidate for the bio-isoprene production, while its thermal instability remains as a challenge. Enzyme engineering efforts, such as direction evolution or semi-rational evolution, are planned for further research.</AbstractText><CopyrightInformation>Copyright © 2018 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Meijie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: li_mj@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Changqing</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: liucq@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Hailin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: chenhl@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Deng</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: dengli@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Haibo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: zhanghb@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nian</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: nianrui@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xian</LastName><ForeName>Mo</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China. Electronic address: xianmo1@qibebt.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>09</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Biosci Bioeng</MedlineTA><NlmUniqueID>100888800</NlmUniqueID><ISSNLinking>1347-4421</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.-</RegistryNumber><NameOfSubstance UI="D019883">Alkyl and Aryl Transferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.-</RegistryNumber><NameOfSubstance UI="C093854">isoprene synthase</NameOfSubstance></Chemical><Chemical><RegistryNumber>I38ZP9992A</RegistryNumber><NameOfSubstance UI="D008274">Magnesium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D019883" MajorTopicYN="N">Alkyl and Aryl Transferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004795" MajorTopicYN="N">Enzyme Stability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020450" MajorTopicYN="N">Heavy Ions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027723" MajorTopicYN="N">Ipomoea batatas</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008274" MajorTopicYN="N">Magnesium</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Characterization</Keyword><Keyword MajorTopicYN="N">Enzyme</Keyword><Keyword MajorTopicYN="N">Ipomoea batatas</Keyword><Keyword MajorTopicYN="N">Isoprene synthase</Keyword><Keyword MajorTopicYN="N">Thermostability</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>03</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>07</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30190176</ArticleId><ArticleId IdType="pii">S1389-1723(18)30239-1</ArticleId><ArticleId IdType="doi">10.1016/j.jbiosc.2018.07.022</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Meijie" sort="Li, Meijie" uniqKey="Li M" first="Meijie" last="Li">Meijie Li</name></noRegion><name sortKey="Chen, Hailin" sort="Chen, Hailin" uniqKey="Chen H" first="Hailin" last="Chen">Hailin Chen</name><name sortKey="Deng, Li" sort="Deng, Li" uniqKey="Deng L" first="Li" last="Deng">Li Deng</name><name sortKey="Liu, Changqing" sort="Liu, Changqing" uniqKey="Liu C" first="Changqing" last="Liu">Changqing Liu</name><name sortKey="Nian, Rui" sort="Nian, Rui" uniqKey="Nian R" first="Rui" last="Nian">Rui Nian</name><name sortKey="Xian, Mo" sort="Xian, Mo" uniqKey="Xian M" first="Mo" last="Xian">Mo Xian</name><name sortKey="Zhang, Haibo" sort="Zhang, Haibo" uniqKey="Zhang H" first="Haibo" last="Zhang">Haibo Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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